MINISTRY OF EDUCATION
MINISTRY OF AGRICULTURE
AND TRAINING
AND RURAL DEVELOPMENT
VIETNAM ACADEMY OF AGRICULTURAL SCIENCES
*******
TRAN THI PHUONG HANH
MAIZE HYBRID BREEDING FOR HIGH YIELD AND RUST
(puccinia sp.) TOLERANCE IN THE
CENTRAL HIGHLANDS
Major Field: Genetics and Plant Breeding
Code: 62.62.01.11
DOCTORAL THESIS SUMMARY
Ha Noi - 2015
The Doctoral thesis was completed in:
VIETNAM ACADEMY OF AGRICULTURAL SCIENCES
Scientific Supervisors:
1: Dr. Bui Manh Cuong
2: Dr. Tran Quang Tan
Reviewer 1:
Reviewer 2:
Reviewer 3:
The thesis is defended at Council for Thesis Assessment at University/Institutional
level, held at:
Vietnam Academy of Agricultural Sciences
At
hours
day
month
year
The Ph.D thesis may be found at:
Vietnam National Library
The Library of Vietnam Academy of Agricultural Sciences
The Library of National Maize Resarch Institute
1
INTRODUCTION
1. THESIS URGENCY
Maize (Zea may L.) is considered as one of the most important cereal crops in the
world and the second after rice in Vietnam.
The Central Highlands is of soil and climate very favorable for maize. However, maize
cultivars still don’t fully express its potential yield due to drought and diseases, especially
rust (Puccinia sp.). Annually, there may be of 15 - 20% loss in the first crop season and
about 25 - 40%, even up to 60% in the second ones.
In order to explore potential and advantages of the Central Highlands, it is necessary to
use high yield and rust tolerant maize hybrids to improve maize yield and production for
the Central Highlands. From reasons mentioned above, It would be urgent to carry out the
thesis “ Maize hybrid breeding for high yield and rust (puccinia sp.) tolerance in the
central highlands”
2. RESEARCH OBJECTIVES
Developing inbred lines and selecting some promising crosses with high yield,
tolerance to rust and suitability for ecological conditions and cultivation habits in the
Central Highlands.
3. Scientific and practical significances
Scientific significance
Providing data, scientific facts and applicability of materials in breeding maize hybrids
with high yield, rust tolerance for the Central Highlands
Practical significance
- Selecting a nursery of materials including 28 inbred lines with high yield and rust
tolerance in the Central Highlands;
- Introducing some promissing crosses: VN5885 recognized as trial production,
VN665 and VN667 tested in the official testing system with high yield, rust tolerance for
maize production in the Central Highlands, contributing into improving maize yield and
production in this region.
2
4. MATERIALS AND SCOPE OF RESEARCH
* Materials
- Including inbred lines derived from commercial hybrids such as NK67, NK66, C919,
CP888, CP999, P4097, DeKalbgold, Pacific 747, LVN10 and LVN4; top-croses, dialell
crosses, promissing crosses; local checks: LVN4, LVN99, LVN885, C919, DK9901,
CP888, NK67.
- Rust disease on maize.
* Scopes
- Experiments of evaluating lines and crosses, genetic diversity using SSR markers;
- Evaluating combing ability, heterosis, adaptability, stability of crosses in the Central
Highlands and other ecological zones.
5. NEW CONTRIBUTIONS OF THE THESIS
The research in the dissertation found a nursery consisting of 28 inbred lines with rust
tolerance as materials for developing high yield hybrids with rust tolerance and developing
a new hybrid namely VN5885, of wide adaptability, early medium maturity, hard stalks,
especially good rust tolerance that recognized as trial production in 2013 by The Ministry
of Agriculture and Rural Development (Decision No. 627/QĐ-TT-CLT dated 30 Dec,
2013) and tested with two promising crosses (VN665 and VN667) in the Central
Highlands and other ecological zones.
6. STRUCTURE OF THE THESIS
The thesis consists of 133 pages, 37 tables, 15 pictures presented in 5 sections:
Introduction (3 pages); Chapter 1: Scientific basis and review of literature (42 pages);
Chapter 2: Materials, contents and research methods (13 pages); Chapter 3: Research
results and discussions (61 pages); Conclusions and suggesions (1 page); References
including 142 documents with 27 in Vietnamse, 109 in English and 6 from websites. There
are two publications related to the dissertation in domestic journals.
3
CHAPTER I
SCIENTIFIC BASIS AND OVERVIEWS
1.1. SCIENTIFIC BASIS
The Central Highlands is very potential zone for agricultural development and the
second major maize area in the country. Maize is mainly used for livestock and partly food
for the ethnic minorities here.
However, maize production in the Central Highlands is still inadequate and
unsustainable, because most of maize areas are mountainous with highly steep slopes,
rainfed condition and low intensive farming. Moreover, due to warm climate, high rainfall
and humidity, it is very favorable for development of diseases harmful to maize especially
rust, which causes major damage on maize in the Central Highlands (loss of production
annually from 15-20 % in the summer - autumn, and 25- 40% even up to 60% in some
areas in autumn- winter crop season). In addition, current maize cultivars still are not good
at tolerance to diseases so not able to meet requirements of maize development in the
Central Highlands.
Thus, drought and diseases especially rust are major reasons for the reducted maize
production in the Central Highlands. Therefore, it is necessary to have a set of maize
hybrids with high yield, early maturity, rust tolerance etc in order to decrease the losses of
maize production for the Central Highlands.
1.2. FOREIGN RESEARCH WORKS RELATED TO THE THESIS
1.2.1. Maize production and usage in the world
Due to important role in the global economy, there should always be great interests in
and more and more continuous development of maize production. In 2001, globle maize
area is 140.2 million ha with an average yield of 4.3 tons.ha-1 and an output of over 600
million tons. The proportion of maize area accounts for 20 % of the total area of cereal
crops. The level of average annual growth in maize production worldwide during 2000 2010 is 1.8 % of area, 2.1 % of yield and 4.3% of production. By 2013, corn area in the
world is 184.19 million ha with average yield of 5.52 tons.ha-1 and production of more
than 1016.74 million tons.
4
1.2.2. Heterosis and its application in maize breeding
Heterosis is defined as the superiority of a hybrid cross over its two parents in vitality,
adaptability, productivity and quality.
Until now, heterosis has been researched in details, divided into five types: heterosis of
morphology, productivity, adaptability, early maturity and physiology, biochemistry.
1.2.3. Inbred lines, methods of selection and evaluation
1.2.3.1. Definition of inbred lines
Inbred lines are relative concepts to refer to self-pollinated lines at a level of high homozygosity
and genetic stability in many traits. For maize, it takes 7-9 self-fertilized generations to obtain
inbred lines.
1.2.3.2. Methods of inbred line development
Selfing, sib-mating (sib or fullsib), additivo accumulative, selection of similar lines,
backcross, etc. Besides, some methods of new line development such as gamete selection, DH
lines via anther or unfertilized ovule culture, inducers use, etc.
1.2.3.3. Combing ability
Combination ability is an inherited characteristic, passed down progenies through
selfing and crossing. Combination ability is determined by evaluating general and specific
combining ability.
1.2.3.4. Evaluation of combing ability: Top-crossing, dialell-crossing
1.2.4. Genetic diversity and its implications into maize hybrid breeding
Maize is typically a cross pollinated plant, very diverse populations and heterozygous
individuals. So the database about the genetic diversity of germplasm is essential and
extremely useful in the evaluation of lines, determination of heterotic groups and
prediction of promissing high yield test-crosses.
To evaluate genetic diversity, there are many methods such as morphological markers,
biochemical markers, DNA molecular markers.
1.2.5. Rust disease on maize
1.2.5.1. Pathogens: Puccinia sorghi, Puccinia polysora, Physopella zeae.
5
1.2.5.2. The environmental influence on the infection and development of the disease
The infection as well as the dispersity of Puccinia sorghi and Puccinia polysora is
affected by environmental conditions.
Favourable temperature: Puccinia sorghi from 15 to 25°C, Puccinia polysora between
23 and 28°C.
1.2.5.3. The influence of rust disease on maize: causing a reduction or loss in
photosynthetic capacity, an increase in respiratory rate, strong evaporation, water
transportation interruption, resulting in the loss of plant water balance, making plants
withered and reducing grain yield.
1.2.5.4. The variety of pathogens
Various biological types of Puccinia sorghi, which causes impacts on hosts, are
calculated in the amount of spores dispersing on sensitive seedlings. For Puccinia
polysora, there are at least 10 races and known as EA1, EA2, EA3, PP.3, PP.4, PP.5, PP.6,
PP.7 and PP.8, PP.9, PP.10.
1.2.5.5. The inheritance of rust resistance in maize
Qualitative resistance is controled by single dominant genes. Rust resistance by
Puccinia polysora: 11 genes (Rpp1 - Rpp11). Rust resistance by Puccinia sorghi: 25 Rp
dominant genes.
Quantitative resistance: polygene traits and inheritance to the next generation is in
accordance with additive principles and of high general combining ability.
1.2.5.6. The management of rust in maize
In order to control rust resistance in corn, there should be with resistant varieties,
chemical measures and cultivation methods.
1.2.5.7. The study of breeding maize varieties with high yield and rust tolerance
Worldwide, there have been many research works on rust and breeding high-yielding
maize varieties resistant to rust. These studies are generally carried out by traditional
methods, mainly in India. Recently, the combination of traditional methods and
biotechnology also has identified a number of high-yielding maize cultivars resistant to
rust. However, these studies are still of limitation.
6
1.3. DOMESTIC RESEARCHES RELATED TO THE THESIS
1.3.1. Maize production and usage in Vietnam
Maize hybrids have played a major role in an increase in maize productivity and yield
in Vietnam. During 1960 - 1980, maize yield of Vietnam only reached 0.8 -1.1 tons.ha-1
due to using local varieties and obsolete cultivation techniques. In 1990, applying
improved maize varieties, grain yield gained 1.5 tons.ha-1. In 1991, maize hybrid area
accounted for only less than 1% of 447,000 ha. In 2000, hybrids accounted for 65% of
total maize acreage and contributed in improving average yield of 2.75 tons.ha-1. In 2013,
the maize area is 1.17 million ha, of which hybrids are more than 95%. The success in
hybrid maize program has significantly contributed in increasing nationwide average yield
of 4.44 tons.ha-1.
1.3.2. Maize production in the Central Highlands
The Central Highlands is of 243.9 thousand ha maize, accounting for 20.6% maize
area. In 2013, maize area of the Central Highlands was 252.4 thousand ha, mainly in three
provinces DakLak (123.4 thousand ha), GiaLai (52.6 thousand ha) and DakNong (52.9
thousand ha). Maize yield in the region is 51.7 quintals.ha-1, ranked third among maize
regions in Vietnam, higher than the nationwide average yield (44.4 quintals.ha-1) and
equivalent to the world average (55.2 quintals.ha-1). Due to an increase in area and
productivity, maize production in the region has been increased by 7.56% compared to in
2012, with 1,306.1 thousand tons and 25.14% of national production.
1.3.3. Inbred lines and evaluation
In Vietnam, since 1990, it has focused on making inbred lines to develop hybrids.
Until now, there have been many inbred lines with high yield made by traditional methods.
In addition, many high-yielding inbred lines has been also created by appying
biotechnology.
1.3.4 Genetic diversity and its implications in maize breeding
In Vietnam, based on morphological criteria, a number of authors have studied the
genetic diversity of maize germplasm. DNA molecular markers have also been used to
7
support the development of hybrids such as using RAPD, SSR markers in analyzing the
genetic diversity of line nursery, clustering and predicting heterosis as well.
1.3.5. Researches on rust and breeding high-yielding maize varieties tolerant to rust
In Vietnam, researches on maize rust are still limited and until now there have been
have two determined species of rust fungi on maize. These are Puccinia polysora and
Puccinia sorghi.
This dissertation is the first work of maize hybrid breeding for high yield and rust
(puccinia sp.) tolerance in the central highlands in Vietnam.
CHAPTER 2
MATERIALS AND METHODS
2.1. RESEARCH MATERIALS
The inbred lines derived from commercial hybrids such as NK67, NK66, C919, CP888,
CP999, P4097, DeKalbgold, Pacific 747, LVN10 and LVN4; Checks in the experiments of
test-cross selection as NK67 and C919: Checks used in official testing, breeding testing are
NK67, C919, DK9901, CP888, LVN4, LVN885, LVN99.
29
SSR
primers
from
Invitrogen
company
announced
at
website:
http://www.maizegdb.org/ssr.php
2.2. RESEARCH CONTENTS
- Investigation of damage on corn caused by rust in the Central Highlands;
- Evaluation of agronomical characteristics, tolerance of rust tolerant lines;
- Evaluation of genetic diversity, genetic purity and combing ability of the nursery of
rust tolerant lines;
- Evaluation of agronomical characteristics, tolerance, heterosis of top-crosses, dialell
crosses, and selection of promising crosses;
- Testing of maize hybrids tolerant to rust in the Central Highlands and other
ecological zones.
8
2.3. METHODOLOGY
2.3.1. Investigating methods on the production and diseases on corn
• Interview
• Method of assessment and investigation in field
Applying methods of research, investigation and detection of diseases under “Plant
protection Research Methods” of the Institute of Plant Protection, 2003.
2.3.2. Methods of artificial infection
According to the method of artificial infection by Meena Shekhar, Sangit Kumar and
Nguyen Thi Binh (1990).
2.3.3. Method of assessing lines, top-crosses and dialell crosses
Evaluating agronomical characteristics, tolerance, yield of lines and crosses as well;
and data collection under the guidance of CIMMYT (1985);
Evaluating top-crosses, dialell crosses according to the model of Griffing 4 (1956).
2.3.4 Methods of assessing genetic diversity by SSR markers
Evaluating genetic diversity under the guidance of AMBIONET-CIMMYT (2004)
2.3.5. Testing methods
Applying the procedures for maize testing No.10 BC-341-2006 by The Ministry of
Agriculture and Rural Development
2.3.6. Data processing
The experimental results processed by the program Excel 5.0, MSTAT 4.0, SAS 9.1;
Combining ability for yield in top crosses and dialell crosses in according to model
Griffing 4 analyzed by Nguyen Dinh Hien’s Diallel program version 2.0; Tree
genealogical mapping with specific software NTSYS pc version 2.1.
2.4. LOCATIONS AND TIME
• Time: from 2009 to 2014
- During 2009-2011: Investigating of damage on corn caused by rust in the Central
Highlands; Having selected a nursery of rust tolerant lines, evaluated the genetic diversity
by molecular markers, developed the combing ability by top-crossing and dialell -crossing,
tested hybrid crosses.
- During 2011-2014: Having evaluated the growth and development of some promising
crosses.
• Locations: in the Central Highlands and Hanoi.
9
CHAPTER 3
RESULTS AND DISCUSSION
3.1. DAMAGES CAUSED BY RUST ON MAIZE IN THE CENTRAL HIGHLANDS
3.1.1. Current status of diseases harmful to maize in the Central Highlands
To assess the prevalence of harmful diseases on maize in the field, conducting surveys
at summer-autumn and autumn-winter crop seasons in 2009 in the Central Highlands
showed that most of diseases such as rust, sheath blight, northern corn leaf blight, southern
corn leaf blight and stalk rot are popular on maize. The prevalence of these diseases is only
5 - 25% in Autumn-Winter, but much higher in the Autumn-Winter crop season with 25 50%), in which rust is the most remarkable.
3.1.2. Current status of maize rust in the Central Highlands
Rust disease often takes place in Autumn-Winter rather than in Summer-Autumn crop
season. Depending on local areas but the prevelance is from 5 to 25% in Summer-Autumn
while in the range of about 25 - 50% in Autumn-Winter crop season. Rust disease is a main
cause of reducing maize yield in the Central Highlands. The losses caused by rust are
estimated as about 14.44% in summer-autumn and 27.46% in autumn-winter crop seasons
and depend on each cultivar. Therefore, it should be necessary to have a set of high-yielding
maize cultivars with rust tolerance for reducing yield loss in the Central Highlands.
3.2.
AGRONOMICAL
CHARACTERISTICS,
TOLERANCE
OF
RUST
TOLERANT LINES
3.2.1. Results of selecting a nursery of rust tolerant lines for the Central Highlands
Through evaluating tolerance ability of 40 lines via artificial infection as well as
accessment in field, it showed that there are 28 lines expressing good rust tolerance as B67a,
B67c, M67a, M67b, G2, G3, G17, G31, G40, G41, G43, G45, G46, G47, G286, G288,
G289, G1234, G1235, G1237, G1238, C4N, C10N, C2N, C3N, C90N, C89N, C88N. These
lines may be used as materials for high yield and rust tolerance maize hybrid breeding in the
Central Highlands.
3.2.2. Agronomical characteristics and tolerance of the nursery of rust tolerant lines
Evaluation results on agronomical characteristics and tolerance of lines in Autumn
10
Winter season 2009 at two locations: Dan Phuong - Ha Noi and Buon Ma Thuot - DakLak.
It should be presented in table 3.7 while yield components and yield in the Central
Highlands are shown in Table 3.11
Table 3.7. Tolerance ability of studied lines
No.
1
2
3
4
5
6
7
8
9
10
11
12
13
14
15
16
17
18
19
20
21
22
23
24
25
26
27
28
29
30
Name of
lines
Lodging
ĐP
B67a
1
B67c
1
M67a
3
M67b
3
G2
2
G3
2
G17
2
G31
2
G40
2
G41
2
G43
2
G45
2
G46
2
G47
2
G286
3
G288
3
G289
3
G1234
3
G1235
3
G1237
3
G1238
3
C2N
2
C3N
2
C4N
3
C10N
1
C89N
3
C90N
3
C88N
2
T5 (Check)
1
DF2 (Check) 3
BMT
1
1
2
2
1
1
1
2
2
2
1
1
1
1
2
2
2
2
2
2
2
2
2
3
1
3
3
2
1
2
Tolerance ability (1- 5)
Drought
Leaf blight Sheath blight
Rust
tolerrance
ĐP BMT ĐP BMT ĐP BMT ĐP BMT
1
1
2
2
2
2
1
2
1
1
2
2
2
2
1
2
2
3
2
3
2
3
1
2
2
3
2
3
2
3
1
2
2
2
1
2
1
2
1
2
1
2
1
2
1
2
1
2
2
2
2
3
2
3
2
2
2
2
2
3
2
3
2
2
2
2
1
2
2
3
2
2
2
2
1
2
2
3
2
2
2
2
1
2
2
2
1
2
2
2
2
2
1
2
1
1
2
2
2
2
1
2
1
2
2
2
1
2
2
2
1
1
2
3
1
1
2
3
1
2
2
2
1
1
2
3
1
1
2
3
1
3
2
3
1
1
2
1
1
1
2
2
1
1
2
2
1
1
2
2
1
1
2
1
1
2
1
2
1
1
2
2
2
3
1
2
1
1
3
3
3
3
3
3
2
2
3
3
3
3
3
3
2
2
3
3
2
2
1
1
2
2
2
2
2
2
1
1
1
1
2
2
1
2
2
2
3
3
2
2
1
2
1
2
2
2
2
2
1
2
1
1
2
2
1
1
2
2
1
1
1
1
2
3
2
3
2
3
3
3
Notice: Point 1-5: best - worst
DP: Dan Phuong and BMT: Buon Ma Thuot
11
Table 3.11. Yield components and grain yield of lines in BuonMaThuot- DakLak
Name Ear Length
No.
of lines
Cm CV%
1 B67a 12.8 7.3
2 B67c 13.3 6.1
3 M67a 14.3 7.0
4 M67b 16.1 8.3
5
G2 12.8 7.2
6
G3 12.8 6.1
7 G17 12.6 3.7
8 G31 14.0 6.1
9 G40 14.8 7.3
10 G41 12.1 6.1
11 G43 12.8 5.8
12 G45 13.0 5.8
13 G46 13.6 6.3
14 G47 12.0 4.3
15 G286 13.8 5.6
16 G288 13.6 11.4
17 G289 12.7 10.7
18 G1234 12.3 8.7
19 G1235 13.8 5.8
20 G1237 13.6 8.0
21 G1238 12.7 6.7
22 C2N 11.3 10.2
23 C3N 11.2 5.8
24 C4N 14.7 6.1
25 C10N 14.6 3.7
26 C89N 13.7 5.8
27 C90N 14.3 6.3
28 C88N 14.3 4.5
T5
29
13.5 6.1
(Check)
DF2
30
14.4 5.6
(Check)
CV%
LSD 0.05
Ear
Diameter
Cm CV%
3.4 4.6
3.4 5.6
3.8 4.3
3.8 6.3
3.4 5.1
3.6 6.6
3.2 7.6
3.6 10.2
3.4 8.2
3.7 6.1
3.3 8.3
3.6 8.3
3.6 10.6
3.4 4.5
3.3 5.8
3.2 6.2
3.6 3.3
3.7 6.6
3.8 5.8
4.0 7.3
3.9 6.3
4.4 6.3
4.4 4.2
4.4 8.3
4.5 6.7
4.3 5.8
3.8 3.4
4.1 3.7
Rows CV% Kernels
12.0 11.7
22.3
14.0
8.9
28.3
14.0
7.9
21.5
12.0 14.4
27.1
12.0 10.2
25.9
12.0 13.5
24.0
12.0 12.4
21.0
12.0
8.6
27.6
12.0
10
25.2
12.0
8.6
25.6
12.0
8.3
25.5
14.0 10.0
27.1
12.0 10.2
24.0
12.0 14.1
20.6
12.0
8.3
23.6
12.0
3.9
27.1
14.0
4.0
22.0
14.0
7.8
24.9
14.0
8.6
21.6
14.0
7.1
22.3
14.8
5.8
20.4
14.8
3.3
21.9
14.4
3.7
27.1
13.6
6.1
32.7
14.0
7.3
28.1
12.0 10.2
30.0
12.0
7.1
26.1
12.0
6.6
27.3
3.7
8.0
12.0
8.6
22.8
7.6
261.7
24.7
3.1
6.3
12.0
8.2
25.3
4.8
233.5
22.4
Kernel rows
Kernels per row
CV%
13.9
4.5
12.6
10.5
4.9
10.7
17.3
13.6
8.9
11.4
10.4
4.9
12.7
14.1
8.9
5.4
8.5
15.0
12.9
6.9
11.4
5.7
8.2
6.6
5.4
6.7
8.1
9.1
P.1000
Yield
kernels
(Quintal/ha)
(g)
239.3
23.6
242.0
24.8
258.0
38.6
260.0
32.5
242.0
28.6
257.6
29.8
252.5
28.3
240.0
30.0
252.6
30.7
248.6
32.4
243.3
35.6
242.2
36.6
243.3
33.3
251.1
34.7
256.8
29.6
244.9
36.3
254.0
34.5
244.0
30.3
234.5
34.7
242.0
27.6
237.6
30.7
310.0
32.1
299.1
30.4
333.7
36.3
334.1
38.5
310.2
36.5
293.6
35.5
285.5
30.3
6.48
3.331
In Autumn- Winter 2009
The results of the overall assessment of agronomical characteristics and tolerance
show that selected 28 lines for development of early mature varieties with rust
12
tolerance and maturity (108 -118 days in the Central Highlands, 112-106 days in
Northern Vietnam), which are of healthy seedling stage, uniformity in morphological
traits, good resistance especially rust (point 1 - 2) and quite high yield (23.6 to 36.6
quintals.ha-1), meet the requirements of the program on developing rust tolerant maize
hybrid for the Central Highlands.
3.3. GENETIC DIVERSITY AND COMBINING ABILITY OF THE NURSERY OF
RUST TOLERANT MAIZE LINES
3.3.1 Genetic diversity of the nursery of rust tolerant maize lines
All of lines with rust tolerance ability are satisfactory to the requirements in the study of
genetic diversity, to the proportion of homozygous genotypes more than 80%. PIC value of 29
studied loci which is in the range of 0.124 to 0.809 and of 0.523 on average demonstrates that
it is quite diverse in the level of genetic diversity in the nursery of studied lines. Through the
tree genealogical chart of studied lines, it should be indicated that these 30 lines are clearly
divided into two groups at the degree of similarity as 0.272 (27.2%).
- Group I: including 22 lines and 4 subgroups as following:
Subgroup 1.1: 8 lines, consisting of G1238, G1237, M67a, M67c, G31, G3, G17 and G2.
Subgroup 1.2: 3 lines as G40, G41 and G43.
Subgroup 1.3: 9 lines, these are G45, G46, G47, G1234, G1235, G286, G288, G289
and T5.
Subgroup 1.4: 2 lines, B67a and B67c.
- Group II: including 6 lines: C4N, C89N, C2N, C3N, C90N and C88N.
- C10N and the check DF2 are classified separately from the rest of the lines at the
degree of similarity as 0.21 (21%).
Based on the results of genetic clustering with 29 SSR loci combined with the results
of an assessment of agronomical characteristics, these lines were divided into two sets of
experiments in order to make an evaluation of combining ability of yield:
- Experiment 1: Mainly consisting of lines of group I (21 lines, excluding line T5) and
line DF2, using top-cross method because of a large number of lines;
- Experiment 2: Including lines of group II, C10N and T5 lines, applying Griffing 4
13
diagram of dialell- crossing.
Evaluating results are presented in the next sections.
G1238
G1237
M67a
M67b
G31
G3
G17
G2
G40
G41
G43
G45
G46
G47
G1234
G1235
G286
G288
G289
T5
B67a
B67c
C4N
C89N
C2N
C3N
C90N
C88N
DF2
C10N
G1238
0.20
0.38
0.56
0.74
0.92
Coefficient
Figure 3.8. Tree genealogical chart on genetic relationship of 30 lines based on the
analysis of 29 SSR loci
3.3.2. Combining ability of rust tolerant line nursery for grain yield
The experiment on evaluating combining ability was implemented in summer-autumn
season 2010 at two locations: Cu M'gar and Buon Ma Thuot.
3.3.2.1. Combining ability evaluation of rust tolerant line nursery for grain yield by
top-crossing
The results of assessing combining ability of 20 lines by top-crossing with 2 testers B67a
and G2 should be represented in table 3.14.
14
Table 3.14. Combining ability of 20 experimental lines for grain yield
General
No.
Name of lines
Specific combing ability
combining
ability
Variance of specific
Tester 1 (B67a)
Tester 2 (G2)
combing ability
1
M67a
4.574
-4.523
4.522
40.906
2
M67b
-1.209
0.994
-0.994
1.977
3
G3
-19.976
0.527
-0.528
0.557
4
G17
-10.526
-8.256
8.256
136.318
5
G31
-7.492
-12.856
12.856
330.545
6
G40
-10.326
11.111
-11.111
246.901
7
G41
-13.742
7.094
-7.094
100.654
8
G43
-9.659
3.177
-3.178
20.193
9
G45
4.208
-2.256
2.256
10.178
10
G46
14.258
2.027
-2.028
8.222
11
G47
9.441
-0.989
0.989
1.957
12
G286
10.158
3.627
-3.628
26.318
13
G288
1.141
0.344
-0.344
0.237
14
G289
-10.059
2.644
-2.644
13.983
15
G1234
5.724
-4.139
4.139
34.265
16
G1235
1.658
-2.539
2.539
12.895
17
G1237
10.524
1.261
-1.261
3.179
18
G1238
10.641
1.777
-1.778
6.319
19
B67c
9.208
-1.089
1.089
2.373
20
DF2
1.458
2.061
-2.061
8.494
In summer-autumn crop season 2010 at Cu M'gar
3.3.2.2. Combining ability evaluation for grain yield by dialell-crossing
The results of evaluating the combining ability of 8 lines by dialell-crossing are shown
15
in table 3.15.
Table 3.15: Combining ability of 8 experimental lines for grain yield
Variance of combing ability
No.
Name of lines
General combining ability (gi)
1
C3N
2.838
11.525
2
C2N
-4.340
44.234
3
C4N
5.665
37.054
4
C10N
5.010
3.126
5
C88N
-5.951
20.457
6
C89N
-8.474
69.422
7
T5
5.854
24.773
8
C90N
-0.601
82.073
(si2)
In summer-autumn season 2010 at Buon Ma Thuot
Based on the results of line evaluation on agronomical traits, tolerance and combing
ability for grain yield, it should be determined that some lines are of maturity, agronomical
traits, tolerance satisfactory to the objectives of the thesis, in which 11 lines carry precious
traits such as rust tolerance, high general combining ability, high variance of specific
combining ability for grain yield, these are G2, G45, G46, G47, G286, G1237, G1238, B67a,
B67c, C4N and C10N.
3.4. THE RESULTS OF EVALUATING AGRONOMICAL TRAITS, TOLERANT
ABILITY, HETEROSIS OF TOP CROSSES, DIALELL CROSSES AND
SELECTION OF PROMISSING CROSSES
Testing top-crosses and dialell crosses was carried out in summer-autumn season 2010
at 2 locations Cu M'gar and Buon Ma Thuot with two checks NK67 and C919.
3.4.1. The evaluation of agronomical traits, tolerant ability, hetorosis of top-crosses
The tolerant ability of top-crosses should be presented in table 3.17.
16
Table 3.17. Tolerant ability of top-crosses (scale of 1-5)
No.
Names of crosses
1
2
3
4
5
6
7
8
9
10
11
12
13
14
15
16
17
18
19
20
21
22
23
24
25
26
27
28
29
30
31
32
33
34
35
36
37
38
39
40
41
42
M67a
X
M67b
X
G3
X
G17
X
G31
X
G40
X
G41
X
G43
X
G45
X
G46
X
G47
X
G286
X
G288
X
G289
X
G1234 X
G1235 X
G1237 X
G1238 X
B67c
X
DF2
X
M67a
X
M67b
X
G3
X
G17
X
G31
X
G40
X
G41
X
G43
X
G45
X
G46
X
G47
X
G286
X
G288
X
G289
X
G1234 X
G1235 X
G1237 X
G1238 X
B67c
X
DF2
X
NK67
C919
B67a
B67a
B67a
B67a
B67a
B67a
B67a
B67a
B67a
B67a
B67a
B67a
B67a
B67a
B67a
B67a
B67a
B67a
B67a
B67a
G2
G2
G2
G2
G2
G2
G2
G2
G2
G2
G2
G2
G2
G2
G2
G2
G2
G2
G2
G2
Rust
Sheath Blight
3
2
2
2
3
2
1
2
3
1
2
1
1
2
1
2
2
2
2
2
2
2
2
2
3
1
2
3
2
1
2
3
1
2
2
2
2
2
2
2
2
2
2
2
1
2
2
2
2
1
2
2
2
2
2
1
2
2
2
2
1
1
1
2
2
1
2
1
1
3
2
2
2
2
1
2
2
3
2
2
1
2
1
2
Northern corn Southern corn
leaf blight
leaf blight
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
2
2
3
2
2
1
2
1
2
1
2
1
2
2
2
2
2
1
2
2
2
1
2
1
2
1
2
1
2
2
2
1
2
2
2
1
2
1
2
2
3
2
2
1
2
2
2
2
2
1
2
2
3
2
2
1
2
2
2
2
2
2
3
2
2
2
3
2
2
Notice: Point 1-5: best – worst
In summer-autumn season 2010 at Cu M'gar
Grain yield and yield heterosis of top crosses are available in table 3.19.
17
Table 3.19. Grain yield and yield heterosis of top crosses
No.
1
2
3
4
5
6
7
8
9
10
11
12
13
14
15
16
17
18
19
20
21
22
23
24
25
26
27
28
29
30
31
32
33
34
35
36
37
38
39
40
41
42
Name of lines
M67a
M67b
G3
G17
G31
G40
G41
G43
G45
G46
G47
G286
G288
G289
G1234
G1235
G1237
G1238
B67c
DF2
M67a
M67b
G3
G17
G31
G40
G41
G43
G45
G46
G47
G286
G288
G289
G1234
G1235
G1237
G1238
B67c
DF2
X
X
X
X
X
X
X
X
X
X
X
X
X
X
X
X
X
X
X
X
X
X
X
X
X
X
X
X
X
X
X
X
X
X
X
X
X
X
X
X
NK67
C919
CV%
LSD0.05
B67a
B67a
B67a
B67a
B67a
B67a
B67a
B67a
B67a
B67a
B67a
B67a
B67a
B67a
B67a
B67a
B67a
B67a
B67a
B67a
G2
G2
G2
G2
G2
G2
G2
G2
G2
G2
G2
G2
G2
G2
G2
G2
G2
G2
G2
G2
Grain yield
(Quintals/ha)
76.7
76.4
57.2
57.9
56.3
77.4
70.0
70.2
78.6
92.9
85.1
90.4
78.1
69.2
78.2
75.8
88.4
89.1
84.8
80.2
88.8
77.5
59.2
77.4
85.1
58.3
58.9
66.9
86.2
91.9
90.1
86.2
80.5
67.0
89.6
83.9
89.0
88.6
90.0
79.1
76.4
73.2
3.87
4.903
Hmp (%)
143.1
157.9
95.4
92.9
92.3
172.2
139.9
119.3
146.7
200.9
176.6
205.9
155.5
115.7
158.6
159.2
191.4
188.7
201.7
181.4
177.6
157.8
99.3
154.4
186.3
101.7
98.8
106.2
166.8
193.6
188.9
187.4
159.5
105.9
191.9
182.8
189.0
183.1
215.4
173.5
Notice: Hmp- Average heterosis; Hs - Standard heterosis;
In summer-autumn crop season 2010 at Cu M’gar
Hs (%)
(NK67)
0.4
0.0
-25.1
-24.3
-26.3
1.4
-8.4
-8.2
2.9
21.6
11.4
18.4
2.3
-9.4
2.4
-0.8
15.8
16.6
11.0
4.9
16.2
1.4
-22.5
1.4
11.3
-23.7
-22.9
-12.5
12.8
20.3
18.0
12.9
5.4
-12.3
17.2
9.8
16.4
15.9
17.8
3.5
Hs (%)
(C919)
4.8
4.4
-21.9
-20.9
-23.1
5.8
-4.4
-4.1
7.4
27.0
16.3
23.5
6.7
-5.4
6.9
3.5
20.8
21.7
15.8
9.5
21.3
5.9
-19.1
5.8
16.2
-20.4
-19.6
-8.7
17.7
25.6
23.1
17.8
10.0
-8.5
22.4
14.6
21.5
21.0
23.0
8.0
18
Based on the results of testing top-crosses in experiment 1, it must be found that two
crosses G46 x B67a and G46 x G2 are of precious agronomical traits such as time early
medium maturity, compact plants, long cylindrical ears, quite good tolerance, especially rust
(point 1), yields more than 90 quintals/ha higher than checks NK67 and C919.
3.4.2. Evaluation of tolerance ability, heterosis of dialell- crosses
The tolerance ability of of crosses is presented in table 3.21.
Table 3.21. The tolerance ability of of crosses (scale of 1 – 5)
No.
Names of crosses
Rust
Sheath Blight
1
2
3
4
5
6
7
8
9
10
11
12
13
14
15
16
17
18
19
20
21
22
23
24
25
26
27
28
29
30
C3N x C2N
C3N x C10N
C3N x C4N
C3N x C88N
C3N x C89N
C3N x T5
C3N x C90N
C2N x C10N
C2N x C4N
C2N x C88N
C2N x C89N
C2N x T5
C2N x C90N
C10N x C4N
C10N x C88N
C10N x C89N
C10N x T5
C10N x C90N
C4N x C88N
C4N x C89N
C4N x T5
C4N x C90N
C88N x C89N
C88N x T5
C88N x C90N
C89N x T5
C89N x C90N
T5 x C90N
NK67
C919
1
1
2
1
1
2
2
2
1
2
2
1
2
1
1
1
1
1
1
2
2
2
2
1
1
2
2
2
2
3
1
1
2
2
2
2
2
2
2
2
2
1
2
2
3
3
3
3
3
2
2
2
2
2
2
2
3
3
2
2
Northern corn
leaf blight
1
2
2
2
1
2
2
2
2
1
2
1
2
1
2
2
2
2
2
2
2
2
2
3
1
2
3
3
2
1
Southern corn
leaf blight
1
2
2
2
2
2
2
2
2
2
2
1
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
Notice: point 1-5: Best - Worst
In summer-autumn crop season 2010 at Buon Ma Thuot
Grain yield and heterosis for yield of dialell crosses are shown in table 3.23.
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